Assistant Researcher
M_MED-ZSFG-PULM
My research focusses on the causative agent of tuberculosis, Mycobacterium tuberculosis: I apply a combination of wet work and bioinformatics to investigate genomic and transcriptional associates of drug resistance, clinical outcomes, and patient infectiousness. See www.SemiQuant.com for more information.
Publications
Bedaquiline resistance in patients with Xpert MTB/RIF Ultra-tested rifampicin-resistant tuberculosis in the Western Cape, South Africa: a prospective study.
The Lancet. Microbe
CountFU: Ditching CFUs for qPCR enumeration of Mycobacterium tuberculosis and a call to join the revolution.
Tuberculosis (Edinburgh, Scotland)
Mycobacterial DNA Extraction using Bead Beating in Custom Buffer Followed by NGS Workflow.
Journal of visualized experiments : JoVE
Matrix-based DNA Extraction for Targeted Next-Generation Sequencing on Decontaminated Sputum Samples.
Journal of visualized experiments : JoVE
stilPCR increases the effective sequencing length of Illumina targeted next-generation sequencing.
Research Square
Mycobacterium tuberculosis resides in lysosome-poor monocyte-derived lung cells during chronic infection.
Journal of acquired immune deficiency syndromes (1999)
selSeq: A method for the enrichment of non-polyadenylated RNAs including enhancer and long non-coding RNAs for sequencing.
Research Square
Anaerobe-enriched gut microbiota predicts pro-inflammatory responses in pulmonary tuberculosis.
D_Cell and Tissue Biology
Bacterial and host determinants of cough aerosol culture positivity in patients with drug-resistant versus drug-susceptible tuberculosis.
P_Pharmaceutical Chemistry
Differential RD-1-specific IFN-γ host responses to diverse Mycobacterium tuberculosis strains in HIV-uninfected persons may be explained by genotypic variation in the ESX-1 region.
Frontiers in reproductive health
A landscape of genomic alterations at the root of a near-untreatable tuberculosis epidemic.
Public health
Linezolid interruption in patients with fluoroquinolone-resistant tuberculosis receiving a bedaquiline-based treatment regimen.
Frontiers in reproductive health
Outcomes, infectiousness, and transmission dynamics of patients with extensively drug-resistant tuberculosis and home-discharged patients with programmatically incurable tuberculosis: a prospective cohort study.
M_Epidemiology & Biostatistics
Xpert MTB/RIF Results in Patients With Previous Tuberculosis: Can We Distinguish True From False Positive Results?
The American journal of tropical medicine and hygiene