Matthias Wabl, PhD

Professor
Microbiology and Immunology

I hold a Ph.D. from the Max-Planck Institute in Berlin. Before joining the UCSF Faculty, I was a Member of the Basel Institute for Immunology, Basel, Switzerland, and a Principal Investigator at the Max-Planck Institute in Tübingen, Germany. I contributed to the molecular genetics of antibody synthesis, including the development of tadpoles from single B lymphocytes; the demonstration that the immunoglobulin class switch occurs within an antigen specific clone by a looping-out and deletion mechanism of DNA; and that subversion of DNA mismatch repair contributed to affinity maturation of antibodies. I also con­struc­ted one of the first bispecific antibodies, and I discovered one of the first mammalian chaperones (BiP).

After generating a human antibody transgenic mouse (the Trianni Mouse) for the discovery of fully human antibodies, I decided to use this platform to combat infectious disease—specifically tuberculosis. We generate antibodies to surface structures and secreted proteins of mycobacterium tuberculosis and test them and their derivatives (bispecifics and other modifications) for their potential use in human therapy of drug resistant tuberculosis.

Publications: 

Infectivity and insertional mutagenesis of endogenous retrovirus in autoimmune NZB and B/W mice.

The Journal of general virology

Beck-Engeser GB, Ahrends T, Knittel G, Wabl R, Metzner M, Eilat D, Wabl M

APOBEC3 enzymes restrict marginal zone B cells.

European journal of immunology

Beck-Engeser GB, Winkelmann R, Wheeler ML, Shansab M, Yu P, Wünsche S, Walchhütter A, Metzner M, Vettermann C, Eilat D, DeFranco A, Jäck HM, Wabl M

Insertional hypermutation in mineral oil-induced plasmacytomas.

European journal of immunology

Knittel G, Metzner M, Beck-Engeser G, Kan A, Ahrends T, Eilat D, Huppi K, Wabl M

Gene expression profiling of murine T-cell lymphoblastic lymphoma identifies deregulation of S-phase initiating genes.

Leukemia research

Dabrowska MJ, Ejegod D, Lassen LB, Johnsen HE, Wabl M, Pedersen FS, Dybkær K

Dysregulated RasGRP1 responds to cytokine receptor input in T cell leukemogenesis.

Science signaling

Hartzell C, Ksionda O, Lemmens E, Coakley K, Yang M, Dail M, Harvey RC, Govern C, Bakker J, Lenstra TL, Ammon K, Boeter A, Winter SS, Loh M, Shannon K, Chakraborty AK, Wabl M, Roose JP

B cell tolerance and positive selection in lupus.

Journal of immunology (Baltimore, Md. : 1950)

Eilat D, Wabl M

Psoriasis patients are enriched for genetic variants that protect against HIV-1 disease.

PLoS genetics

Chen H, Hayashi G, Lai OY, Dilthey A, Kuebler PJ, Wong TV, Martin MP, Fernandez Vina MA, McVean G, Wabl M, Leslie KS, Maurer T, Martin JN, Deeks SG, Carrington M, Bowcock AM, Nixon DF, Liao W

Sequential class switching is required for the generation of high affinity IgE antibodies.

The Journal of experimental medicine

Xiong H, Dolpady J, Wabl M, Curotto de Lafaille MA, Lafaille JJ

CD22 serves as a receptor for soluble IgM.

European journal of immunology

Adachi T, Harumiya S, Takematsu H, Kozutsumi Y, Wabl M, Fujimoto M, Tedder TF

An autoimmune disease prevented by anti-retroviral drugs.

Retrovirology

Beck-Engeser GB, Eilat D, Wabl M

Pro-B cells sense productive immunoglobulin heavy chain rearrangement irrespective of polypeptide production.

Proceedings of the National Academy of Sciences of the United States of America

Lutz J, Heideman MR, Roth E, van den Berk P, Müller W, Raman C, Wabl M, Jacobs H, Jäck HM

Overbuilding: overhead revisions.

Science (New York, N.Y.)

Wabl M

Orphan receptor GPR110, an oncogene overexpressed in lung and prostate cancer.

BMC cancer

Lum AM, Wang BB, Beck-Engeser GB, Li L, Channa N, Wabl M

Early onset of autoimmune disease by the retroviral integrase inhibitor raltegravir.

Proceedings of the National Academy of Sciences of the United States of America

Beck-Engeser GB, Eilat D, Harrer T, Jäck HM, Wabl M

Activation of the brain-specific neurogranin gene in murine T-cell lymphomas by proviral insertional mutagenesis.

Gene

Nielsen AA, Kjartansdóttir KR, Rasmussen MH, Sørensen AB, Wang B, Wabl M, Pedersen FS

Immunosuppressive human anti-lymphocyte autoantibodies specific for the type 1 sphingosine 1-phosphate receptor.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Liao JJ, Huang MC, Fast K, Gundling K, Yadav M, Van Brocklyn JR, Wabl MR, Goetzl EJ

Expression of Aire and the early wave of apoptosis in spermatogenesis.

Journal of immunology (Baltimore, Md. : 1950)

Schaller CE, Wang CL, Beck-Engeser G, Goss L, Scott HS, Anderson MS, Wabl M

Pvt1-encoded microRNAs in oncogenesis.

Retrovirology

Beck-Engeser GB, Lum AM, Huppi K, Caplen NJ, Wang BB, Wabl M

Differentiation and Ig-allele switch in cell line WEHI-231.

Journal of immunology (Baltimore, Md. : 1950)

Spillmann FJ, Beck-Engeser G, Wabl M

Ig heavy chain promotes mature B cell survival in the absence of light chain.

Journal of immunology (Baltimore, Md. : 1950)

Geraldes P, Rebrovich M, Herrmann K, Wong J, Jäck HM, Wabl M, Cascalho M

Impairment of alternative splice sites defining a novel gammaretroviral exon within gag modifies the oncogenic properties of Akv murine leukemia virus.

Retrovirology

Sørensen AB, Lund AH, Kunder S, Quintanilla-Martinez L, Schmidt J, Wang B, Wabl M, Pedersen FS

Retroviral activation of the mir-106a microRNA cistron in T lymphoma.

Retrovirology

Lum AM, Wang BB, Li L, Channa N, Bartha G, Wabl M

Activation of an oncogenic microRNA cistron by provirus integration.

Proceedings of the National Academy of Sciences of the United States of America

Wang CL, Wang BB, Bartha G, Li L, Channa N, Klinger M, Killeen N, Wabl M

A tumor-suppressor function for NFATc3 in T-cell lymphomagenesis by murine leukemia virus.

Blood

Glud SZ, Sørensen AB, Andrulis M, Wang B, Kondo E, Jessen R, Krenacs L, Stelkovics E, Wabl M, Serfling E, Palmetshofer A, Pedersen FS

Somatic hypermutation and mismatch repair in non-B cells.

European journal of immunology

Klasen M, Spillmann FJ, Marra G, Cejka P, Wabl M

Hypermutation rate normalized by chronological time.

Journal of immunology (Baltimore, Md. : 1950)

Wang CL, Wabl M

Retroviral vectors to monitor somatic hypermutation.

Journal of immunological methods

Klasen M, Spillmann FJ, Lorens JB, Wabl M

Mutational activity in cell line WEHI-231.

Immunogenetics

Wang CL, Wabl M

Directed molecular evolution by somatic hypermutation.

Protein engineering, design & selection : PEDS

Wang CL, Yang DC, Wabl M

Precise dosage of an endogenous mutagen in the immune system.

Cell cycle (Georgetown, Tex.)

Wang CL, Wabl M

Endogenous expression of activation-induced cytidine deaminase in cell line WEHI-231.

Journal of immunology (Baltimore, Md. : 1950)

Spillmann FJ, Wabl M

DNA acrobats of the Ig class switch.

Journal of immunology (Baltimore, Md. : 1950)

Wang CL, Wabl M

Genome-wide somatic hypermutation.

Proceedings of the National Academy of Sciences of the United States of America

Wang CL, Harper RA, Wabl M

DNA-PKcs function regulated specifically by protein phosphatase 5.

Proceedings of the National Academy of Sciences of the United States of America

Wechsler T, Chen BP, Harper R, Morotomi-Yano K, Huang BC, Meek K, Cleaver JE, Chen DJ, Wabl M

The possibility of B-cell-dependent T-cell development.

Scandinavian journal of immunology

Keshavarzi S, Rietz C, Simões S, Shih S, Platt JL, Wong J, Wabl M, Cascalho M

Contribution of light chain rearrangement in peripheral B cells to the generation of high-affinity antibodies.

European journal of immunology

Magari M, Sawatari T, Kawano Y, Cascalho M, Wabl M, Kanayama N, Hikida M, Ohmori H

SWAP-70-deficient mast cells are impaired in development and IgE-mediated degranulation.

European journal of immunology

Gross B, Borggrefe T, Wabl M, Sivalenka RR, Bennett M, Rossi AB, Jessberger R

Impaired IgE response in SWAP-70-deficient mice.

European journal of immunology

Borggrefe T, Keshavarzi S, Gross B, Wabl M, Jessberger R

VH gene replacement in thymocytes.

Journal of immunology (Baltimore, Md. : 1950)

Golub R, Martin D, Bertrand FE, Cascalho M, Wabl M, Wu GE

B cell immunopoiesis: visualizing the impact of CD40 engagement on the course of T cell-independent immune responses in an Ig transgenic system.

European journal of immunology

Erickson LD, Vogel LA, Cascalho M, Wong J, Wabl M, Durell BG, Noelle RJ

Specific antibody production by V(H)-gene replacement.

European journal of immunology

Madan MK, Golub R, Wabl M, Wu GE

Association of SWAP-70 with the B cell antigen receptor complex.

Proceedings of the National Academy of Sciences of the United States of America

Masat L, Caldwell J, Armstrong R, Khoshnevisan H, Jessberger R, Herndier B, Wabl M, Ferrick D

Germinal centers without T cells.

The Journal of experimental medicine

de Vinuesa CG, Cook MC, Ball J, Drew M, Sunners Y, Cascalho M, Wabl M, Klaus GG, MacLennan IC

A B220(-), CD19(-) population of B cells in the peripheral blood of quasimonoclonal mice.

International immunology

Cascalho M, Wong J, Brown J, Jäck HM, Steinberg C, Wabl M

Mapping of the SWAP70 gene to mouse chromosome 7 and human chromosome 11p15.

Immunogenetics

Masat L, Liddell RA, Mock BA, Kuo WL, Jessberger R, Wabl M, Morse HC

Dendritic cells associated with plasmablast survival.

European journal of immunology

García De Vinuesa C, Gulbranson-Judge A, Khan M, O'Leary P, Cascalho M, Wabl M, Klaus GG, Owen MJ, MacLennan IC

Secondary rearrangements and hypermutation generate sufficient B cell diversity to mount protective antiviral immunoglobulin responses.

The Journal of experimental medicine

López-Macías C, Kalinke U, Cascalho M, Wabl M, Hengartner H, Zinkernagel RM, Lamarre A

Cellular, intracellular, and developmental expression patterns of murine SWAP-70.

European journal of immunology

Borggrefe T, Masat L, Wabl M, Riwar B, Cattoretti G, Jessberger R

Hypermutation in antibody affinity maturation.

Current opinion in immunology

Wabl M, Cascalho M, Steinberg C

A mouse with a monoclonal primary immunoglobulin repertoire not further diversified by V-gene replacement.

Developmental immunology

Cascalho M, Martin DA, Wong J, Lam Q, Wabl M, Wu GE

A B-cell-specific DNA recombination complex.

The Journal of biological chemistry

Borggrefe T, Wabl M, Akhmedov AT, Jessberger R

The Ig mutator is dependent on the presence, position, and orientation of the large intron enhancer.

Proceedings of the National Academy of Sciences of the United States of America

Bachl J, Olsson C, Chitkara N, Wabl M

Mismatch repair co-opted by hypermutation.

Science (New York, N.Y.)

Cascalho M, Wong J, Steinberg C, Wabl M

VH gene replacement in hyperselected B cells of the quasimonoclonal mouse.

Journal of immunology (Baltimore, Md. : 1950)

Cascalho M, Wong J, Wabl M

Critical test of hot spot motifs for immunoglobulin hypermutation.

European journal of immunology

Bachl J, Steinberg C, Wabl M

A protein binding specifically to the IgG2b switch region.

Developmental immunology

Völk H, Wabl M

Loss of the beta 1 subunit of the sodium pump during lymphocyte differentiation.

European journal of immunology

Masat L, Cascalho M, Wabl M

A quasi-monoclonal mouse.

Science (New York, N.Y.)

Cascalho M, Ma A, Lee S, Masat L, Wabl M

Translatable immunoglobulin germ-line transcript.

European journal of immunology

Bachl J, Turck CW, Wabl M

Affinity maturation and class switching.

Current opinion in immunology

Wabl M, Steinberg C

An immunoglobulin mutator that targets G.C base pairs.

Proceedings of the National Academy of Sciences of the United States of America

Bachl J, Wabl M

Biochemical studies of class switch recombination.

Current topics in microbiology and immunology

Jessberger R, Wabl M, Borggrefe T

Enhancers of hypermutation.

Immunogenetics

Bachl J, Wabl M

Somatic hypermutability.

Current topics in microbiology and immunology

Wabl M, Steinberg C

A rapid assay for detecting cellular TdT enzymatic activity.

Journal of immunological methods

Harriman W, Fischer ER, Wabl M

Large cytoplasmic inclusion body kappa-chain has unusual intrachain disulfide bonding.

Journal of immunology (Baltimore, Md. : 1950)

Jäck HM, Sloan B, Grisham G, Reason D, Wabl M

Immunoglobulin class switch recombination.

Annual review of immunology

Harriman W, Völk H, Defranoux N, Wabl M

A different sort of Mott cell.

Proceedings of the National Academy of Sciences of the United States of America

Jäck HM, Beck-Engeser G, Sloan B, Wong ML, Wabl M

Allelic exclusion model questioned.

Nature

Wabl M, Steinberg C

Tumorigenesis mediated by an antigen receptor.

Proceedings of the National Academy of Sciences of the United States of America

Jäck HM, Beck-Engeser G, Lee G, Wofsy D, Wabl M

The murine IgG1/IgE class switch program.

European journal of immunology

Siebenkotten G, Esser C, Wabl M, Radbruch A

HeLa-LAV, an epithelial cell line stably infected with HIV-1.

Journal of virological methods

Berg J, Doe B, Steimer KS, Wabl M

Bispecific antibodies that mediate killing of cells infected with human immunodeficiency virus of any strain.

Proceedings of the National Academy of Sciences of the United States of America

Berg J, Lötscher E, Steimer KS, Capon DJ, Baenziger J, Jäck HM, Wabl M

Beswitched. The looping out model for immunoglobulin class switching.

The New biologist

von Schwedler U, Jäck HM, Wabl M

Immunoglobulin lambda gene rearrangement can precede kappa gene rearrangement.

Developmental immunology

Berg J, McDowell M, Jäck HM, Wabl M

Translation affects immunoglobulin mRNA stability.

European journal of immunology

Jäck HM, Berg J, Wabl M

Scope of action of the immunoglobulin mutator system.

Genome

Wabl MR, Jäck HM, von Borstel RC, Steinberg CM

Looping out and deletion mechanism for the immunoglobulin heavy-chain class switch.

Proceedings of the National Academy of Sciences of the United States of America

Jäck HM, McDowell M, Steinberg CM, Wabl M

High rates of deletions in the constant region segment of the immunoglobulin mu gene.

Proceedings of the National Academy of Sciences of the United States of America

Jäck HM, Wabl M

Measurements of mutation rates in B lymphocytes.

Immunological reviews

Wabl M, Jäck HM, Meyer J, Beck-Engeser G, von Borstel RC, Steinberg CM

Allelic inclusion in a pre-B-cell line that generates immunoglobulin heavy chain genes in vitro.

Proceedings of the National Academy of Sciences of the United States of America

Beck-Engeser G, Jäck HM, Wabl M

High rate of somatic point mutation in vitro in and near the variable-region segment of an immunoglobulin heavy chain gene.

Proceedings of the National Academy of Sciences of the United States of America

Meyer J, Jäck HM, Ellis N, Wabl M

V lambda 2 rearranges with all functional J lambda segments in the mouse.

European journal of immunology

Weiss S, Meyer J, Wabl MR

Hypermutation at the immunoglobulin heavy chain locus in a pre-B-cell line.

Proceedings of the National Academy of Sciences of the United States of America

Wabl M, Burrows PD, von Gabain A, Steinberg C

Immunoglobulin heavy chain toxicity in plasma cells is neutralized by fusion to pre-B cells.

Proceedings of the National Academy of Sciences of the United States of America

Haas IG, Wabl MR

A Mott cell hybridoma.

European journal of immunology

Weiss S, Burrows PD, Meyer J, Wabl MR

Expression of immunoglobulin heavy chain at a high level in the absence of a proposed immunoglobulin enhancer element in cis.

Proceedings of the National Academy of Sciences of the United States of America

Wabl MR, Burrows PD

Allelic inclusion in the pre-B-cell line 18-81.

Proceedings of the National Academy of Sciences of the United States of America

Wabl MR, Beck-Engeser GB, Burrows PD

A theory of allelic and isotypic exclusion for immunoglobulin genes.

Proceedings of the National Academy of Sciences of the United States of America

Wabl M, Steinberg C

Allelic exclusion of immunoglobulin expression is not caused by somatic segregation.

Proceedings of the National Academy of Sciences of the United States of America

Wabl MR, Tenkhoff M

Expression of mu and gamma immunoglobulin heavy chains in different cells of a cloned mouse lymphoid line.

Proceedings of the National Academy of Sciences of the United States of America

Burrows PD, Beck GB, Wabl MR

Simultaneous expression of mouse immunoglobulins M and D is determined by the same homolog of chromosome 12.

Proceedings of the National Academy of Sciences of the United States of America

Wabl MR, Johnson JP, Haas IG, Tenkhoff M, Meo T, Inan R